Medical Lasers; Engineering, Basic Research, and Clinical Application 2020; 9(2): 119-125  
Effect of Photobiomodulation on the Mesenchymal Stem Cells
Shin Hyuk Yoo1,2
1Department of Otorhinolaryngology, Dankook University College of Medicine, Cheonan, Korea
2Institute of Tissue Regeneration Engineering (ITREN), Dankook University, Cheonan, Korea
Correspondence to: Shin Hyuk Yoo
Department of Otorhinolaryngology, Dankook University College of Medicine, 119 Dandae-ro, Dongnam-gu, Cheonan 31116, Korea
Tel.: +82-41-550-3933
Fax: +82-41-556-1090
E-mail: shyoomd@gmail.com
Received: June 4, 2020; Accepted: July 20, 2020; Published online: December 31, 2020.
© Korean Society for Laser Medicine and Surgery. All rights reserved.

Abstract
Photobiomodulation forms the basis of photomedicine and is defined as the effect of coherent or non-coherent light sources, such as low-level lasers and light-emitting diodes, on cells and tissues. This treatment technique affects cell functions, proliferation, and migration, and plays an important role in tissue regeneration. Mesenchymal stem cells (MSCs) are known to be beneficial for tissue regeneration, and the combination of stem cell therapy and laser therapy appears to positively affect treatment outcomes. In general, a low-power laser has a positive effect on MSCs, thereby facilitating improvements in different disease models. This study elucidates the mechanisms and effects of low-power laser irradiation on the proliferation, migration, and differentiation of various MSCs that have been examined in different studies.
Keywords: Photobiomodulation; Mesenchymal stem cell; Laser; Low-level lasers
Introduction

Stem cells are unspecialized, immature cells with self-renewal features that provide a cell source for tissue regeneration and replacement of damaged organs. 1 Differentiation is a critical cellular stage for these cells that allows them to provide specialized cells to form different tissues or organs. Growth factors, extracellular matrices, and cell types are useful in tissue engineering because they increase stem cell differentiation to other cells. 2

Mesenchymal stem cells (MSCs) are mesenchymal stromal cells, regardless of their tissue origin; they were originally found in bone marrow. However, MSCs can be harvested from other adult tissues. 3 These pluripotent cells can differentiate into osteocytes, chondrocytes, and adipocytes. 4 While MSCs can be isolated from many organs, the umbilical cord, umbilical cord blood, and amniotic fluid are the major perinatal MSC sources. Many postnatal organs can be sources of MSCs, including the skin, adipose tissue, blood vessels, and dental pulp. 5

Photobiomodulation (PBM) can be used to promote cell differentiation and expansion. Furthermore, lasers were almost immediately used in medical applications after their invention. Photomedicine is the study and application of light in health care and disease treatment 6 and has been used in dermatology, surgery, intervention radiology, ophthalmology, cardiology, and oncology. PBM therapy promotes cell growth, regeneration, and healing of tissue by means of light sources such as light emitting diodes (LEDs) and low-level lasers (LLL), or other light sources that emit light in the visible red to near-infrared (NIR) range. 7

Several basic in vitro and in vivo studies have examined the effects of PBM, which can be either inhibitory or stimulatory for pain control and regarded as anti-inflammatory effects, or considered to be metabolic or immunological. 8 PBM affects the activity of endogenous enzyme photoacceptors for the initiation of cell signaling pathways. This alters cell and tissue metabolism and cell proliferation. However, the mechanism of the therapeutic laser effect remains poorly understood with respect to its cellular and molecular effects. 9 The present paper aims to review the effects of low-power laser irradiation on the proliferation, migration, and differentiation of different types of MSCs that have been examined in previous studies reported in the literature.

Molecular mechanisms of PBM

TPKR/Ras/Raf/MEK/ERK/Mnk1/eIF4E/CyclinD1 pathway

Several studies have shown that low-power lasers can induce tyrosine-protein kinase receptor (TPKR) such as c-MET that can activate the mitogen-activated protein kinases (MAPK), thereby inducing cell proliferation. This was originally called ERK or extracellular signal-regulated kinase signaling pathway. Eukaryotic initiation factor 4E (eIF4E) is a major regulator of cap-dependent mRNA that responds to various stimuli such as hormones, growth factors, and mitogens. Low-level laser irradiation can also phosphorylate protein heat and acid-stable (PHAS-1) and upregulate the expression of eIF4E and CyclinD, and also increase the proliferation of cells. 10,11

TPKR/PI3K/Akt/mTOR/eIF4E pathway

Phosphoinositide 3-kinase (PI3K) phosphorylation is the most important downstream TPKR pathway. LLL can increase the phosphorylation of Akt and the PI3K pathway, inducing the phosphorylation of PHAS-1 through the phosphorylation of mammalian target of rapamycin (mTOR). 12,13 It activates proliferation and cell migration through eIF4E phosphorylation.

TPKR/PLC-gamma/PKC pathway

The activation of phospholipase C (PLC) can catalyze phospholipids, thereby increasing the concentration of diacylglycerol (DAG) and inositol trisphosphate (IP3). 14 IP3 can increase the levels of calcium from the endoplasmic reticulum, activating protein kinase C (PKC). PKC is effective in cell proliferation, differentiation, and apoptosis. The application of LLL increased the calcium level, thereby promoting cell proliferation.

PI3K/Akt/eNOS pathway

Nitric oxide (NO) can promote angiogenesis and the endothelial nitric oxide synthase (eNOS) signaling pathway can result in a low level of NO. The application of a low-power laser (632.5 nm) can enhance eNOS expression in endothelial cells, promoting proliferation and migration of endothelial cell migration, which is important in angiogenesis. 7,15

ATP/cAMP/JNK/AP-1 pathway

The PBM through LLL can result in an increase in cyclic adenosine monophosphate (cAMP) and consequently c-Jun N-terminal kinase (JNK) phosphorylation, increasing activator protein 1 (AP–1). AP–1 enhances the expression of genes involved in proliferation, survival, and angiogenesis. 16,17

MSCS STUDIED TO DATE

MSCs derived from dental pulp

Dental pulp stem cells have fibroblastic morphology and can differentiate into different types of cells. 18 Increased osteogenic differentiation in dental pulp stem cells due to LLL irradiation was demonstrated. 18 In another study, LLL irradiation of cells has been shown to significantly increase osteogenic and odontogenic differentiation as well as the self-renewal and survival of pulpal stem cells. 19

Adipose MSCs

It was shown that the use of a low-power laser (660 nm ± 20 nm, 6 J/cm2, 10 mV/cm2) can increase the levels of angiogenic factors in adipose MSCs, and after transplantation, could differentiate endothelial cells and improve function. 20 This study showed that apoptosis was reduced in adipose MSCs after treatment with a low-power laser. After identification of CD31, CD34, von Willebrand Factor (vWF), and kD markers by immunofluorescence, the differentiation of the MSCs into the endothelial cells was confirmed. 21,22 LLL irradiation (wavelength: 660 nm + 20 nm, 220 V + 22 V, 50 Hz) can increase cell migration by increasing the levels of focal adhesion kinase (FAK) that regulates cell adhesion and migration signals in cells. The studies also showed that the proliferation and viability of MSCs increased. In one study, it was found that the growth factors of the hepatocyte growth factor (HGF) and platelet-derived growth factor (PDGF) were also elevated. 17 The use of a low-power laser (808 nm, 3 J/cm2, 200 mV, 0.2 W/cm2) for seven days and 5 min increased the proliferation and viability of the cells. 23 The effect of a low-power laser (660 nm and 0.5, 1 J/cm2) on adipose and bone marrow-derived MSCs was found to be dose-dependent and led to increased cell growth and proliferation without any nuclear changes. 24 In another study, low-power lasers (660 nm, 550 mW/cm2) accelerated ischemic limb function by accelerating endothelial cell differentiation and secreting growth factors (vascular endothelial growth factor (VEGF), HGF, and fibroblast growth factor (FGF-RRB -). 25

It was reported that the toxic effects of doxorubicin on adipose MSCs can be reduced by low-power laser irradiation (wavelength 660 nm, output power 30 mW, a laser beam of 0.028 cm2, and irradiation of 1.07 mW/cm2). A low-power laser with these characteristics increases the viability of MSCs and inhibits apoptosis and oxidative stress in the MSCs that are treated with doxorubicin. 26 Another study found that a gallium-aluminum-arsenide (GaAlAs) laser (650 nm, 4 J/cm2) improved the repair in a mouse model of skin aging by increasing the proliferation, differentiation, and secretion of the growth factor of the MSCs. Furthermore, the expression of mesenchymal surface markers increased. 27

Bone marrow MSCs

A study showed that a low-power laser (2, 4 J/cm2) significantly increased the proliferation of bone marrow MSCs and differentiated them into osteocytes. 28 Moreover, this study showed that laser irradiation (16 J/cm2) significantly suppressed proliferation and differentiation into the bones. Low-power lasers at the power of 4, 8, and 16 J/cm2 also inhibited the expression of tumor necrosis factor-α (TNF-α). 29 Another study showed that irradiation of bone marrow MSCs by a low-power laser (20 mJ/cm2) differentiated them into neuronal cells. An increased expression of beta-tubulin II protein was also found in this study. 30

It was shown that LLL irradiation applied to the osteoporosis MSCs from ovariectomized mice significantly increased the optical density and cell viability compared to the control group. 31 Another study showed that the effects of a low-power laser strongly depended on the wavelength, the number of laser therapy sessions, the state of MSC physiology, and the type of laser. 32

Many studies have shown that the use of MSCs in the oral maxillofacial area is highly promising. It was shown that the use of a low-power laser (808 nm, 64 J/cm2) increased the expression of important markers for osteoblast differentiation, such as Runt-related transcription factor 2 (RUNX2), alkaline phosphatase (ALP), and osteoblast-specific transcription factor, osterix (OSX). In this study, the reduction of pro-inflammatory factors (IL-6 and IL-17) and an increase in anti-inflammatory cytokines (IL-10 and IL-1) were also observed. 33 In another study, low-power lasers (15 Hz, 150 mJ, 2.25 W) were demonstrated to proliferate and differentiate bone marrow MSCs into osteoblasts in 3-dimensional collagen scaffolds that can be used in the treatment of periodontal diseases. 34

The use of stem cells has been studied as a novel therapeutic approach for the treatment of cardiovascular disease that is one of the leading causes of death worldwide. It was shown that the use of an 810 nm low-power laser significantly reduced the size of the myocardial infarction and significantly increased cell proliferation. 35 Hou et al. found that the use of a low-power laser (0.5J/cm2) promoted the proliferation of MSCs and their myogenic differentiation, while facilitating the secretion of VEGF and nerve growth factor (NGF). 36 In another study, LLL (660 nm, 5 mW, 6, 10, 12 J/cm2) was also shown to significantly increase the proliferation and viability of the bone marrow MSCs in the reactive oxygen species (ROS). 37

Blood MSCs

MSCs can also be used to repair damaged tendons. A study showed that human blood MSCs can be differentiated into tenocytes by LLL. The use of growth factors (epidermal growth factor-2 (EGF2), transforming growth factor beta-3 (TGFB3), insulin-like growth factor-1 (IGF-1), and basic fibroblast growth factor-2 (bFGF2)) with LLL led to the expression of the most important tenogenic genes such as early growth response protein 1 (EGR1), tenascin c (TNC), and decorin (DCN). 38

EFFECT OF DIFFERENT WAVELENGTHS AND LIGHT DOSES ON PBM

A biphasic response to LLL treatment has been demonstrated in several studies. 39,40 The Arndt-Schulz Law has been frequently used as a suitable model to describe the dose-dependent effects of LLL therapy. 41-43 A “biphasic” curve can be used to illustrate the expected dose response to light at a subcellular, cellular, tissue, or clinical level. If insufficient energy is applied, there will be no response because the minimum threshold has not been met. If more energy is applied, the threshold is crossed and biostimulation is achieved; however, when too much energy is applied, the stimulation disappears and is replaced by disinhibition instead.

One study examined the effects of PBM treatment on dental derived MSCs, and the obtained results appeared to follow the Arndt-Schulz law. 44 When the lowest (0.05 J/cm2) and the highest (42 J/cm2) energy densities were applied, no effects were observed. A therapeutic window was observed in the intermediate energy density range (1-4 J/cm2). In this range of energy densities, positive effects of PBM therapy were observed, as demonstrated in literature for other cell types. 45-47

Since wavelength affects tissue penetration, shorter wavelengths (600-700 nm) are considered to be optimal for the treatment of superficial tissue, and longer wavelengths (780 to 950 nm) are preferred for the treatment of deeper tissues. Wavelengths in the 700-770 nm range showed no significant activity. 48 Wu et al. used a 730 nm laser on mice with traumatic brain injuries and found it to be ineffective, while irradiation treatments using 660 and 810 nm lasers were effective. 49 For wound healing in mice, Gupta et al. found that treatments with 660 - and 810 nm lasers were effective, while the treatment with a 730 nm laser was not effective. 50

The combination of two wavelengths provides an additional effect of the PBM. Comparing irradiation at 830 and 685 nm, Mendez and colleagues found that irradiation at 830 nm led to better results. 51 A recent study showed that PBM with irradiation at both 630 and 810 nm significantly stimulated cell viability and decreased apoptosis of human bone marrow MSCs in vitro. 52

CONCLUSIONS

Table 1 summarizes the results of some studies on the effects of LLL on stem cells. Previous studies have shown that PBM increases the differentiation, proliferation, and migration of MSCs. These results depend on factors such as energy density, power output, frequency of radiation, and the types of light source, cell, or medium culture. To obtain favorable results, standardization of parameters in PBM experiments is required. Since PBM has positive effects on biostimulation, proliferation, and differentiation of MSCs, it can be a powerful tool in regenerative treatments.

Table 1 . Results of researches on the effects of photobiomodulation on the stem cells

Type of cellsWavelength (nm)Output power (mW)Laser beam (cm2)Energy density (J/cm2)Irradiation (mW/cm2)Total treatmentReference
Dental MSC660200.0283, 50.714s, 7sDiniz et al.19
Adipose MSC660300.0280.21.07De Lima et al.26
660 ± 20--61010 minPark et al.21
660 ± 203 and 4.5---1 hYin et al.17
808200-30.27 days (5 min)Nurković et al.23
636859.0859.39 min 10 sMvula et al.39
BM MSC-0.252, 4, 1620 sWang et al.29
632.831.561.2378 sFallahnezhad et al.32
Blood MSC660--5-2 minGomiero et al.38

MSC, mesenchymal stem cell; BM, bone marrow.


CONFLICTS OF INTEREST

The authors declare no potential conflicts of interest with respect to the research, authorship, and/or publication of this article.

FINANCIAL DISCLOSURE

The author has no financial interests related to the content of this article. No outside funding was received.

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