Medical Lasers; Engineering, Basic Research, and Clinical Application  
Stability and Effectiveness of Laser and Liquiband Treatments in the Rat Model of Oral Ulcer
Ji Won Kang1,*, Hyun Seok Ryu1,2,*, Celine Abueva1, Phil-Sang Chung1,3, Seung Hoon Woo1,3
1Beckman Laser Institute Korea, Dankook University, Cheonan, Korea
2Interdisciplinary Program for Medical Laser, Dankook University, Cheonan, Korea
3Department of Otorhinolaryngology Head and Neck Surgery, Dankook University School of Medicine, Cheonan, Korea
Correspondence to: Seung Hoon Woo
Department of Otorhinolaryngology Head and Neck Surgery, Dankook University School of Medicine, 201 Manghyang-ro, Dongnam-gu, Cheonan 31116, Korea
Tel.: +82-41-550-1781
Fax: +82-41-550-7837

*The first two authors equally contributed to this work.
Received: March 23, 2021; Accepted: May 7, 2021; Published online: May 27, 2021.
© Korean Society for Laser Medicine and Surgery. All rights reserved.

Background and Objectives
This study was undertaken to assess the stability and efficacy of laser therapy (808 nm), Liquiband (a commercial topical skin adhesive product), and a combination treatment, for application in oral ulcers.
Materials and Methods
The oral ulcer rat animal model was used to determine the efficacy of photobiomodulation, Liquiband, or combination therapy. Ulcers were induced by injecting 60% acetic acid in the oral mucosa. Three days after ulcer confirmation, the chemically induced ulcers were treated with either laser (808 nm), Liquiband, or a combination of both, every two days for 8 days (4 sessions). Combination therapy was performed by first treating with laser followed by application of the Liquiband. Reduction in ulcer area size was subsequently determined, and animals were sacrificed at 5 and 10 days after the last administration, for histological observation of the extracted oral ulcer tissue samples.
In this study, treatment with either laser (808 nm) or commercial Liquiband product as well as the combined treatment significantly enhanced oral ulcer healing in the rat animal model. The effect of laser treatment is mainly attributed to collagen synthesis, whereas application of the Liquiband promotes vascularization and formation of granulation tissue. Our results indicate that further optimization of the combined therapy could synergistically and significantly enhance oral ulcer healing.
Laser or Liquiband treatment of oral ulcer in the rat animal model exert different outcomes, but both methods confirm significant effects relative to the untreated group. The combined treatment group with laser and Liquiband showed marked oral ulcer healing, but further optimization is required to get highly significant results and establish the potential synergistic effect of the combination therapy, as compared to the individual enhancing effects of healing oral ulcers.
Keywords: Oral ulcer; Low-level light therapy; Cyanoacrylate

This Article

Funding Information
  • Hanmi Healthcare Co., Inc

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