Fig. 3. The effect of PBM on cell proliferation of HCE-T cells. (A, B) Cells were seeded onto 96-well at a density of 2,000 cells/well and incubated overnight in a growth medium. Cells underwent by dual PBM treatment at 30 J/cm² (A) once and 10 J/cm² three times (B) then incubated for 24, 48 and 72 h in 5% CO₂ at 37°C. After incubation, 2 mg/ml of MTT solution was added to each well then was replaced by 100 μl DMSO after 4 h. The growth of cells was determined by measuring the absorbance at 570 nm. (C) Cells were seeded onto 11 mmΦ round coverslip in 24-well plate and were underwent PBM treatment at 30 J/cm². After 24 h, cells were fixed by chilled methanol. Fixed cells were blocked with 3% BSA and then incubated with BrdU (1:100) for overnight at 4°C in a humidified chamber. Cells were then incubated in a 1:100 dilution of Alexa650 probed anti-rabbit IgG and observed using confocal laser scanning microscope. The red positive cells were counted under × 40 magnification. Every assay was performed 3 times and the results are expressed as mean ± SD. One-way ANOVA (Tukey test) were used for comparisons between two means.

Med Lasers 2017;6:67~76 https://doi.org/10.25289/ML.2017.6.2.67

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