Fig. 4. The effect of PBM on cell cycle progression of HCE-T cells. Cells were treated with PBM at 30 J/cm² and incubated at 37°C and 5% CO₂ for 24 h. Cells were collected by trypsinization and were incubated with propidium iodide for 15 minutes. The cell cycle distribution and sub-G1 DNA content were determined and analyzed by flow cytometry. The percentages of viable and dead were determined as 10,000 events per sample using an FL-2 filter and compared with control. Every assay was performed 3 times and the results are expressed as mean ± SD. One-way ANOVA (Tukey test) were used for comparisons between two means.

Med Lasers 2017;6:67~76 https://doi.org/10.25289/ML.2017.6.2.67

© Medical Lasers