Med Laser  
Comparison of the 660 nm and 532 nm Wavelengths for Photobiomodulation Therapy on Dermal Wounds in Mice
Hyun Seok Ryu1,2, Seung Hoon Woo1,3
1Beckman Laser Institute Korea, Dankook University, Cheonan, Korea
2Interdisciplinary Program for Medical Laser, Dankook University, Cheonan, Korea
3Department of Otorhinolaryngology Head and Neck Surgery, Dankook University School of Medicine, Cheonan, Korea
Correspondence to: Seung Hoon Woo
Received: March 10, 2022; Accepted: March 15, 2022; Published online: April 21, 2022.
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Background and Objectives
The objective of this study was to compare the effects of photobiomodulation using the 660 nm diode and 532 nm diode-pumped solid-state (DPSS) lasers on dermal wound healing in mice.
Materials and Methods
The dermal wound was induced by a circular incision on the mice's back using a No. 15 surgical blade. The mice were treated with photobiomodulation at an energy density of 4 J/cm2 daily for 10 days before sacrifice. The macroscopic and histological observations of the wound were done on days 3, 7, and 10.
The dermal wound area reduction rates in the diode laser, DPSS laser, and control groups were 34.2 ± 3.5, 33.2 ± 2.4, 24.0 ± 2.7 on the 3rd day, 72.2 ± 2.8, 64.8 ± 3.5, 42.8 ± 5.0 on the 7th day, and 87.2 ± 3.7, 82.2 ± 7.9, 71.4 ± 4.0 on the 10th day (p < 0.05), respectively. Histological analysis showed that the laser treatment improved granulation tissue formation, epithelialization, collagen deposition, and vascularization compared to the control.
Diode and DPSS lasers significantly improved dermal wound healing in mouse models. Further investigations are needed to confirm the mechanisms involved during photobiomodulation on dermal wound healing.
Keywords: Photobiomodulation; Dermal wound healing

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